In this section
@article{Giblin:2026:10.1159/000553655,
author = {Giblin, SP and Moiseanu, VR and Carrington, CJ and Naing, A and Watkins, K and Tsuchiya, T and Kanegasaki, S and Pease, JE},
doi = {10.1159/000553655},
journal = {J Innate Immun},
pages = {1--29},
title = {The small ubiquitin-like modifier SUMO-3 acts as a neutrophil chemoattractant via the chemokine receptors CXCR1 and CXCR2.},
url = {http://dx.doi.org/10.1159/000553655},
year = {2026}
}
TY - JOUR
AB - INTRODUCTION: Small ubiquitin-like modifiers (SUMOs) are small peptides conjugated to proteins during post-translational modification which have been reported to modulate several aspects of the immune system, notably in auto-immune disorders. METHODS: We used a SUMO-based bacterial expression system to create a recombinant protein putatively expressed by Toxocara canis, which we hypothesised might antagonise responses via the chemokine receptor CXCR1. RESULTS: Although our recombinant Toxocara canis protein was devoid of antagonist activity, we serendipitously observed that recombinant SUMO-3 protein had chemotactic activity for CXCR1 transfectants. Further study found that SUMO-3 acted as a full agonist of CXCR1 and the closely related receptor CXCR2, the latter responses ablated by a CXCR2 antagonist. SUMO-3 showed similar efficacy at both receptors but reduced potency when compared to CXCL8, with chemotaxis observed at high nanomolar to micromolar concentrations. In receptor endocytosis assays, SUMO-3 induced internalisation of CXCR1 and CXCR2, with inferior potency and efficacy to CXCL8. Translating our findings to primary cells, a broad range of SUMO-3 concentration gradients were shown to induce the chemotaxis of human neutrophils. Finally, SUMO-3 was found to be released by necrotic cells into the extracellular milieu. CONCLUSIONS: Collectively, our findings suggest that SUMO-3 can induce the chemotaxis of neutrophils via CXCR1 and CXCR2. We postulate that in vivo, release of SUMO-3 from necrotic cells may serve to recruit neutrophils, contributing to tissue homeostasis and the resolution of inflammation.
AU - Giblin,SP
AU - Moiseanu,VR
AU - Carrington,CJ
AU - Naing,A
AU - Watkins,K
AU - Tsuchiya,T
AU - Kanegasaki,S
AU - Pease,JE
DO - 10.1159/000553655
EP - 29
PY - 2026///
SP - 1
TI - The small ubiquitin-like modifier SUMO-3 acts as a neutrophil chemoattractant via the chemokine receptors CXCR1 and CXCR2.
T2 - J Innate Immun
UR - http://dx.doi.org/10.1159/000553655
UR - https://www.ncbi.nlm.nih.gov/pubmed/42475262
ER -