In this section
@article{Hong:2026:10.1002/advs.75222,
author = {Hong, W and Zhang, Z and Li, A and Sun, T and Wu, Y and Vadukul, DM and Jones, D and Li, B and Liu, F and Aprile, FA and Korchev, Y and Gorelik, J and Klenerman, D and Shevchuk, A},
doi = {10.1002/advs.75222},
journal = {Adv Sci (Weinh)},
title = {A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.},
url = {http://dx.doi.org/10.1002/advs.75222},
volume = {13},
year = {2026}
}
TY - JOUR
AB - Live-cell imaging of cell surface topography and intracellular architecture is essential for understanding cellular function. However, conventional approaches often involve trade-offs between resolution, invasiveness, and volumetric coverage. Here, we present an integrated Scanning Ion Conductance Microscope and single-objective Oblique Plane Microscope (SICM-OPM) system that enables simultaneous non-contact topographical imaging and volumetric fluorescence imaging within the same live cell without sample translation. Beyond correlative live imaging, the platform supports nanomechanical mapping with tens-of-nanometers resolution, fluorescence-guided localized molecular delivery via the SICM, and benefits from reduced photobleaching due to light-sheet excitation. We demonstrate this platform's capabilities by visualizing imipramine-induced transverse-tubule (TT) remodeling in live cardiomyocytes, revealing pronounced detubulation of internal TT invaginations while surface TT opening characteristics remain largely preserved, and capturing high-speed correlative volumetric images of clathrin-mediated endocytosis in Cos-7 cells. Additionally, we show precision delivery of fluorescent cargos, including dextrans and α-synuclein, into mammalian cells and diatoms, alongside localized stiffness mapping to evaluate mechanical responses of mammalian cells. We believe this technique opens new avenues for correlative structural, functional, and biophysical studies in live cells, with broad relevance to cell biology, neurodegeneration, and mechanobiology.
AU - Hong,W
AU - Zhang,Z
AU - Li,A
AU - Sun,T
AU - Wu,Y
AU - Vadukul,DM
AU - Jones,D
AU - Li,B
AU - Liu,F
AU - Aprile,FA
AU - Korchev,Y
AU - Gorelik,J
AU - Klenerman,D
AU - Shevchuk,A
DO - 10.1002/advs.75222
PY - 2026///
TI - A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.
T2 - Adv Sci (Weinh)
UR - http://dx.doi.org/10.1002/advs.75222
UR - https://www.ncbi.nlm.nih.gov/pubmed/41954243
VL - 13
ER -