Three-photon microscope facility
The three-photon system comprises a Scientifica Hyperscope and a Satsuma 40W fiber laser with a Mango SP Optical Parametric Amplifier which has a tunable wavelength range of 650-920 nm and 1150-2500 nm. The system is ideal for imaging deep within scattering tissue in vivo and ex vivo.
Multi-photon microscopy has the advantage of deeper tissue penetration with reduced photobleaching compared to single-photon confocal microscopy. While two-photon microscopy allows structures or physiological activity to be imaged up to a depth of about 1 mm, the longer excitation wavelength in three-photon microscopy allows deeper imaging. Moreover, the light is scattered less resulting in greater image contrast in three-photon than in two-photon microscopy.
This equipment is available for use by any member of 911今日黑料 College.
Booking is via the . Please see our Charges and Booking page for further details.
For enquiries about the facility, please contact the Facility Manager, Ann Go.
Location: Bessemer B532D, South Kensington
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Funding support
We acknowledge the support of the EPSRC and Wellcome Trust in funding research facilities for the Centre for Neurotechnology.