HCF4- NIKON AX R MP NSPARC Confocal

GENERAL SPECIFICATIONS

  • A powerful multiphoton, confocal, super-resolution, and single-photon detecting inverted imaging system from NIKON: . It is based on the ECLIPSE Ti2-E main body and supports a maximum Field of View (FOV) of 25 mm, providing an expansive space under the objective to easily accommodate varied specimens and large sample sizes.
  • Scan Head allows users to capture "bigger pictures, fast" by collecting more data per single frame without the need to constantly change objectives. It supports line scanning, bi-direction scanning, and averaging, with a continuously variable zoom ranging from 1X to 1000X.
  • Standard Image Acquisition (Galvano): Engineered for high-definition deep imaging, the Galvano scanner features a pixel size of up to 8192 x 8192 pixels, enabling Nyquist sampling at any magnification to ensure large images are captured without sacrificing a single detail.
  • High-Speed Image Acquisition (Resonant): The resonant scanner captures the rapid dynamics of life with a 2K resolution capable of up to 2048 x 2048 pixels, ensuring fast, gentle, and high-resolution imaging that keeps delicate live samples healthier for longer periods.
  • Super-Resolution & Macro-to-Micro Imaging: The integration of the NSPARC detector allows the system to seamlessly support the entire range of imaging applications—from macro imaging via high-speed image stitching to super-resolution micro imaging—within a single experiment.
  • Live Cell & High-Throughput Imaging: The system provides full support for live tissue and live cell imaging, featuring comprehensive environmental control including temperature regulation and CO2 supply (premixed gas) via the integrated . Additionally, there is the option to perform complex high-throughput imaging of tissue and multi-well plates, fully supported by the system's motorized XY stages and dedicated multi-well plate holders.
  • Time-Resolved Expansion: The system is upgraded with a .

MICROSCOPE OBJECTIVES

  • CFI Plan Apochromat Lambda D 4X, 10X, and 20X.
  •  (NA 1.42, W.D. 0.15mm, spring loaded, FN 25).
  •  (NA 0.95, W.D. 0.93, Water Immersion, FN 25).
  •  (NA 1.15, W.D. 0.60mm, Chromatic correction: 405-950nm).
  •  (NA 1.05, W.D. 0.55, Silicon Immersion, FN 25).
  •  (NA 1.25, W.D. 0.3, Silicon Immersion, FN 25).

ILLUMINATION

  •  featuring a fixed 1045 nm output and a broad tuning range of 680 nm to 1300 nm, delivering >3.5 W of peak power.
  • Six-line Laser Unit combining 405, 445, 488, 514, 561, and 640nm lines.
  • Fluorescence LED Illumination system offering four individually controllable color channels at 385nm, 475nm, 550nm, and 621nm.

DETECTORS

  • AX R MP Scan Head capable of both point scanning for confocal and multiphoton modalities over a 22mm FOV. It incorporates galvano mirrors for high-resolution imaging up to 8192x8192 and resonant mirrors for high-throughput gentle live-cell imaging up to 2048x2048.
  • 4Ch Detector Unit equipped with two tunable detection paths and two filter wheel paths, covering a detection range from 400nm to 920nm.
  • Non-Descanned Detector (NDD) utilizing 4 GaAsP photomultiplier tubes (PMTs) to collect visible light in the EPI direction from IR-excited dyes without confocal sectioning. 
  • Diascopic Detector Unit for transmitted images and Second Harmonic Generation (SHG) signals utilizing an excitation laser up to IR.
  •  Unit utilizing Image Scanning Microscopy (ISM) to improve resolution up to 100nm laterally and 300nm axially using visible and IR lasers.
  • Picoquant 2 Detector FLIM for AX MP, offering comprehensive FLIM, FRET, and FCS capabilities with 2 detection channels, measuring fluorescence lifetimes from 100 ps to microseconds.
  •  and  cameras for standard imaging tasks.

SOFTWARE

  •  for fully automated camera and confocal system control, supporting 6D imaging, Z-stacks, spectral unmixing, stitching, multipoint experiments, and programmable macro generation.
  • NIS-A 2D/3D Deconvolution utilizing both Blind and Non-blind algorithms to improve image clarity.
  • NIS-Elements Module NIS.Ai featuring AI-driven workflows including , , and . 
  • NIS-A FLIM Module and  Software () for dedicated lifetime data acquisition and advanced analysis scripting.
  •  (GA3) and  Editor for creating complex 3D analysis workflows and autonomous experiment design.

ADD-ONS

  • Piezo Stage offering closed-loop control and 500 microns of high-precision Z-axis nanopositioning.
  • Water Immersion Dispenser to supply pure water to the objective tip, facilitating long-term unassisted immersion observation.
  • Perfect Focus System (v4) with a motorized nosepiece featuring an altered notch filter specifically modified for multiphoton use.